Hua Shao

Lecturer

ADDRESS：NO.136 Science Ave, Zhengzhou, China

PHONE：+86-189-9562-1071 (China)

E-mail：shaohua2011@163.com

Websites：http://shaohua2011.blog.163.com

Research Interests：

1. High Yield of Biocatalyst Produced by E.coli

The catalytic performance and expression yield of enzymes have been widely concerned by the scientific community. However, many foreign proteins were insoluble expressed as IB in E.coli which hinders their applications. Although great progress has been made on enzyme engineering in recent years, it is difficult to achieve the synergistic modification of biocatalysts with various properties. Therefore, the coevolution of enzyme activity and solubility is of great significance for the development of high yield recombinant enzymes. The twin-arginine protein transport pathway in E.coli has the function of screening the folding quality of protein substrate, while the mechanism of this process has not yet been resolved. Ongoing research aims to improving enzyme expression in E.coli and understanding how tat system can be applied for the production of high-value biocatalysts.

2. Harnessing the Endogenous CRISPR-Cas System in E.coli
The advent of CRISPR-Cas-based genome-editing technique mediated by Cas9 has extensively used in many labs. However, the expression of a heterologous Cas9 is toxic and results in low transformation efficiency in many organisms. So, repurposing the endogenously encoded CRISPR-Cas systems for genome editing is an attractive alternative. E. coli is a common microorganism in metabolic engineering and synthetic biology. Exploiting a simple and efficient genome-editing method using the endogenous CRISPR-Cas system (type I-E) in E. coli is helpful to genome engineering. Ongoing research aims to activating the endogenous CRISPR-Cas system in E.coli and constructing an ideal recombinant strain for high yield protein expression in E.coli.

Education Background:

9 / 2000 – 7 / 2004, B.S. inBioscience, College of Life Science, Xinyang Normal University, China

9 / 2007 – 7 / 2010, M.S. inBiophysics, Henan Key Lab of Ion Beam Bioengineering, Zhengzhou University, China

9 / 2010 – 12 / 2014, Ph.D. in Microbiology, College of Life Science and Technology, Huazhong University of Science and Technology, China

Teaching and Research Experience

2015 to current, Lecturer, Biochemistry, Zhengzhou University of Light Industry, China

2015 to current, Lab of biocatalysis and biotransformation, Zhengzhou University of Light Industry, China

Publications:

[1]. Hua Shao, Xianmei Hu, Liping Sun, Wenshan Zhou. Gene cloning, expression in E. coli, and in vitro refolding of a lipase from Proteus sp. NH2-2 and its application for biodiesel production. Biotechnology Letters. 2019, 41: 159-169

[2]. Hua Shao, Li Xu, Yunjun Yan. Thermostable lipases from extremely radioresistant bacterium Deinococcus radiodurans: Cloning, expression and biochemical characterization. Journal of Basic Microbiology. 2014, 54(9): 984-995

[3] Hua Shao, Li Xu, Yunjun Yan. Biochemical characterization of a carboxylesterase from the Archaeon Pyrobaculum sp. 1860 and a rational explanation of its substrate specificity and thermostability. International Journal of Molecular Sciences. 2014, 15(9): 16885-16910

[4]. Hua Shao, Li Xu, Yunjun Yan. Isolation and characterization of a thermostable esterase from a metagenomic library. Journal of Industrial Microbiology and Biotechnology. 2013, 40(11), 1211-1222

Research Projects：

1 / 2016 – 12/2018, Mining for novel lipases from metagenomics library of the South China Sea sediment and their structures resolution, National Natural Science Foundation of China (31501420, Host)

1/ 2012 –12/ 2015, High efficient resolution of 2-arylpropionic acid chiral drugs by chimeric lipase from Candida rugosa, National Natural Science Foundation of China (31170078, Participant)